Bis-Tris Propane/HCl Buffer, pH 7.5 [1M] - 250mL
AOA 365715
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Enhance the stability or activity of your research with our comprehensive range of biological buffers.
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O que se Destaca
Detalhes do produto
- Manufactured in an ISO 9001 and 13485 certified facility in the USA
- For research use only. Not for human or animal clinical/therapeutic use.
- Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests under certified 14644 clean room conditions
- Buffer that is useful in the pH 6.3-9.5 range. This wide buffering range is due to its two pKa values, 6.8 (pKa1) and 9.0 (pKa2), being so close. Because of the wide buffering range, particularly down to pH 6 - 7, this buffer has been used to enhance the stability or activity of restriction enzymes, compared to Tris buffer (which is a poor buffer below pH 7.5 and has a comparatively large change in pKa with temperature).
| Nome da Marca | eCon Lab Supply |
| Fabricante | Ciro Mfg |
| Número da Peça | 23.105.59 |
| Componentes Incluídos | Garrafa |
| Peso do Item | 4 onças (113,4 gramas) |
| Marca | eCon-Lab-Supply |
What's in the box
Bottle
Quem Deverá Comprar?
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Biochemical Research
Ideal for researchers conducting experiments requiring a stable pH near 7.5 for biochemical analyses.
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Protein Studies
Suitable for studies involving proteins, as it maintains a favorable environment for protein stability.
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Molecular Biology
Useful in molecular biology protocols that require reliable buffering at a neutral pH.
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Extreme pH Environments
Not suitable for applications needing a pH outside the 6-8 range as stability may be compromised.
DESCRIÇÃO DO PRODUTO
Perguntas e Respostas dos Clientes
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pergunta:
What is Bis-Tris Propane/HCl Buffer used for?
Resposta: Bis-Tris Propane/HCl Buffer is primarily used for biochemical and molecular biology applications, particularly in protein purification and analysis. Its pH stability allows researchers to maintain consistent conditions for protein functions and enzymatic reactions. In practice, this buffer is ideal for preparing protein samples for electrophoresis or chromatography, ensuring accurate and reproducible results in experimental protocols. -
pergunta:
How should Bis-Tris Propane/HCl Buffer be stored?
Resposta: Bis-Tris Propane/HCl Buffer should be stored at room temperature, away from direct sunlight and moisture to maintain its stability. Proper storage conditions ensure that the buffer maintains its intended pH and concentration. For optimal longevity, keep it in a tightly sealed container. This is crucial in laboratory settings, where maintaining buffer integrity can significantly affect experimental outcomes. -
pergunta:
Can Bis-Tris Propane/HCl Buffer be used with different biological samples?
Resposta: Yes, this buffer is versatile and suitable for use with a variety of biological samples including proteins, nucleic acids, and enzymes. Its compatibility allows researchers to use it effectively in diverse applications such as Western blots, electrophoresis, and other analytical techniques. This adaptability makes it a preferred choice in laboratories working with different biological materials. -
pergunta:
What are the benefits of using Bis-Tris Propane/HCl over other buffers?
Resposta: Bis-Tris Propane/HCl offers several advantages, including excellent buffering capacity at a physiological pH range and minimal interference with biological processes. Unlike some other buffers, it does not chelate metal ions, making it particularly suitable for protein studies where metals may be involved. Researchers often prefer it for its reliability in maintaining stable pH during experiments, which is critical for sensitive biological assays. -
pergunta:
Is Bis-Tris Propane/HCl Buffer compatible with all protein types?
Resposta: In general, Bis-Tris Propane/HCl Buffer is compatible with a wide range of proteins, particularly those sensitive to pH variations. It provides an environment that stabilizes proteins and is preferred for many purification and analytical techniques. However, it is always recommended to validate compatibility for specific proteins and experimental setups to ensure optimal results. -
pergunta:
What is the ideal concentration for using Bis-Tris Propane/HCl Buffer?
Resposta: Bis-Tris Propane/HCl Buffer is typically used at a 1M concentration for most applications. This concentration provides effective buffering capacity, maintaining pH stability under varying conditions. In practical scenarios, researchers might dilute this buffer to lower concentrations depending on specific experimental requirements, while still enjoying its robust buffering characteristics. -
pergunta:
Can I use Bis-Tris Propane/HCl Buffer in sensitive enzymatic reactions?
Resposta: Yes, Bis-Tris Propane/HCl Buffer is particularly suitable for sensitive enzymatic reactions due to its minimal inhibitory effects on enzyme activity and effective pH stabilization. This makes it an excellent choice for reactions involving delicate enzymes, as it helps maintain optimal conditions for activity, ensuring reliable and reproducible experimental results. -
pergunta:
What pH adjustments can be made with Bis-Tris Propane/HCl Buffer?
Resposta: Bis-Tris Propane/HCl Buffer can be adjusted to stable pH levels typically between 6.5 and 8.5, making it versatile for various applications. Adjustments can be made using hydrochloric acid or sodium hydroxide. This flexibility allows researchers to tailor the buffer to meet specific experimental conditions, accommodating the unique pH requirements of different biological systems. -
pergunta:
Who should use Bis-Tris Propane/HCl Buffer?
Resposta: Bis-Tris Propane/HCl Buffer is ideal for researchers, students, and professionals in molecular biology, biochemistry, and related fields. Its reliability and effectiveness in maintaining pH make it a vital tool in experimental design. Whether you’re working on protein purification, enzyme assays, or academic research, this buffer can support accurate scientific investigations. -
pergunta:
Where can I buy Bis-Tris Propane/HCl Buffer, pH 7.5 [1M] - 250mL?
Resposta: You can purchase Bis-Tris Propane/HCl Buffer, pH 7.5 [1M] - 250mL from Ubuy in Angola. Ubuy provides a reliable platform with extensive product offerings, making it easy for you to find and order the specific chemicals and lab supplies you need for your research or laboratory work.
eCon Lab Supply Buffers Editorial Review
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AOA 365715
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Recursos e benefícios
- Wide buffering range (pH 6.3-9.5) for versatile applications.
- Manufactured in an ISO 9001 and 13485 certified facility in the USA.
- Strict adherence to Good Manufacturing Practices.
- Quality control tests conducted under certified clean room conditions.
- Ideal for protein research, molecular biology, and characterization studies.
- Not for human or animal clinical/therapeutic use.
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